Friday, August 12, 2016

Summer Research in the Anderson Lab

Niles High School student Madeline Stanton and biochemistry major Victor Gutierrez-Schultz have been working with Professor Gretchen Anderson this summer to correct a mutation in the enzyme, arsenite oxidase. The two genes for the enzyme (one gene for each subunit) were cloned into E. coli by previous IU South Bend students, but along the way, a mutation was introduced in the smaller subunit. Victor analyzed the sequencing data from last year (thanks to 2016 biochemistry graduate Jared LaRue), found the mutation, and used genetic engineering to fix the mutation back to its native nucleotide. Both Madeline and Victor then worked together to confirm that the mutation was indeed fixed, and are now sequencing the two genes to determine if any further mutations were introduced during the fix.